چاپ مقاله Application of Nano-b-Cyclodextrin to Induce Biosynthesis of Phenylpropanoids and Antioxidant Activity of Basil

جهت دانلود مقاله اینجا کلیک کنید

 

Abstract
Metabolic engineering leads to plant productivity by inducing engineered nanoparticles in the metabolites biosynthesis
pathway. In the present study, b-cyclodextrin nanoparticles (b-CDNPs) were synthesized, and their physical and chemical
structure was investigated using transmission electron microscopy (TEM), dynamic light scattering (DLS), and zeta
potential. Afterward, we used b-CDNPs with 0, 10, 50, 100 mg/L concentrations for foliar application of the aerial parts of
basil (Ocimum basilicum L c.v. Keshkeni luvelou). GC-MS was used to identify compounds. b-CDNPs foliar application
with a concentration of 50 ppm stimulated growth and significantly improved the growth indexes. In the presence of bCDNPS, phenylpropanoids concentration, phenol content, and flavonoids total content in the aerial parts of basil showed a
significant difference (P B 5%) compared to the control group. According to molecular analysis, eugenol O-methyltransferase (EOMT) and chavicol O-methyltransferase (CVOMT) genes expression in methyl eugenol and methyl chavicol
biosynthesis pathways were significantly improved when treated with 50 ppm concentration compared to the control. Basil
may show antioxidant activity when treated with different concentrations of b-CDNPs, in a dose-dependent manner. Thus,
b-CDNPS may act as an inducer with their stimulating, maintaining the basil phenylpropanoids as a bio-elicitor with the
potential of free radical scavenging to enhance the basil’s therapeutic and nutritional properties.